Fig. 5
From: Co-targeting WIP1 and PARP induces synthetic lethality in hepatocellular carcinoma
WIP1 and PARP inhibition pronounced DNA damage. A γH2AX and cleaved-PARP1 (C-PARP1) were measured via Western blotting in PLC/PRF/5 cells after WIP1 knockdown or inhibition combined with Olaparib (50 μM, 24 h) treatment. B γH2AX and C-PARP1 was measured via Western blotting to evaluated the DNA damage levels and apoptosis of PLC/PRF/5 cells after WIP1 knockdown or inhibition combined with Veliparib (50 μM, 24 h) treatment. C γH2AX and C-Caspase3 was measured via Western blotting in Hepa1-6 cells after WIP1 inhibition combined with Olaparib (50 μM, 24 h) treatment. D γH2AX and c-PARP1 was measured via Western blotting to evaluated the DNA damage levels and apoptosis of Hepa1-6 cells after WIP1 inhibition combined with Veliparib (50 μM, 24 h) treatment. E The foci of γH2AX was measured via immunofluorescence in PLC/PRF/5 cells after WIP1 knockdown combined with Olaparib (50 μM) or Veliparib (50 μM) treatment for 24 h. And the numbers of foci per cell were counted and shown. F The foci of γH2AX was measured via immunofluorescence to evaluated the DNA damage levels of PLC/PRF/5 cells after GSK2830371(25 μM) combined with Olaparib (50 μM) or Veliparib (50 μM) treatment for 24 h. And the numbers of foci per cell were counted and shown