Fig. 3
From: Co-targeting WIP1 and PARP induces synthetic lethality in hepatocellular carcinoma
WIP1 suppression inhibits HCC development in vivo. Xenograft model (n = 5 per group) was performed with WIP1 stably knockdown Hepa1-6 cells (Hepa1-6-shWIP1), controlled with Hepa1-6-shNC stable cells. And tumor pictures (A), tumor growth curve (B) and tumor weight (C) were shown respectively. D WIP1 protein level in 6 pairs of DEN-induced mice liver cancer tissues and adjacent non-tumor tissues was analyzed by Western blotting. E Representative macroscopic images of WIP1 wild-type (+ / +) or knockout (+ / −) mice with DEN-induced HCC. F The average number of tumors per mouse in DEN-induced HCC (size > 2 mm) in WIP1 wild-type (+ / +) or knockout (+ / −) mice. G The histopathological examination of wild-type (+ / +) or knockout (+ / −) mice liver tissues. H The ki-67 immunohistochemical staining of wild-type (+ / +) or knockout (+ / −) mice liver tissue