Fig. 2
From: Co-targeting WIP1 and PARP induces synthetic lethality in hepatocellular carcinoma
Suppression of WIP1 inhibits proliferation of HCC cells in vitro. A Cell viability of PLC/PRF/5 and Hepa1-6 cells with or without WIP1 knockdown by siRNAs was measured with MTS assay. B Cell viability of HCC-LM3 cells with ectopic overexpression of WIP1 was measured with MTS assay. C Cell viability of PLC/PRF/5 and Hepa1-6 cells treated with WIP1 inhibitor GSK2830371 with indicated concentrations for 72 h was measured with MTS assay. D Representative image of colony formation and quantitative analysis of colony numbers of PLC/PRF/5 and Hepa1-6 cells with WIP1 stable knockdown with shRNA. E Representative image of colony formation and quantitative analysis of colony numbers of PLC/PRF/5 and Hepa1-6 cells with GSK2830371(12.5 μM, 24 h). F The apoptosis of HCC cells with or without WIP1 knockdown with siRNA was assessed via flow cytometry with PI and annexin V–FITC double staining. G The apoptosis of HCC cells with or without GSK2830371 treatment for 72 h was assessed via flow cytometry with PI and annexin V–FITC double staining