Construct | Sequence | |
---|---|---|
Wild type 2 | Forward | 5′-TAGGCTGGTCTTGAACTCCTGGC-3′ |
(− 447/+ 1012) | Reverse | 5′-GACAGCAGTCAGGATCTGGGAGCAGGAG-3′ |
Mut 1 | Forward | 5′-GGAGTATTCGCGCTCACTTGCCTGCCTCTGTCT-3′ |
Reverse | 5′-TGAGCGCGAATACTCCACAAAGTCCACCTGG-3′ | |
Mut 2 | Forward | 5′-GCTGGGCCTTTCTCCCACACCCCCTCCTACCCT-3′ |
Reverse | 5′-GAGAAAGGCCCAGCCCCCGACAGCCAGCGC-3′ | |
Mut 3 | Forward | 5′-TCTGGAAAGGCCCAGGTCTTCTGCGATCAGTTA-3′ |
Reverse | 5′-CCTGGGCCTTTCCAGATTGAAAGGGGTACAGGA-3′ | |
Mut 4 | Forward | 5′-CACATGCGAATAAGACGGAGGAAAAAACAAACA-3′ |
Reverse | 5′-TCTTATTCGCATGTGTGTGTGTGCCATGCAC-3′ | |
Mut 5 | Forward | 5′-ATAGTAAAGGTTGGTATGTATGTGCCTGTGGGT-3′ |
Reverse | 5′-ACCAACCTTTACTATAGCAACAGCGTGTGCAAG-3′ |