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Table 1 Primer sequences of mutant S100A4 promoter used in this study

From: A functional role of S100A4/non-muscle myosin IIA axis for pro-tumorigenic vascular functions in glioblastoma

Construct

Sequence

Wild type 2

Forward

5′-TAGGCTGGTCTTGAACTCCTGGC-3′

(− 447/+ 1012)

Reverse

5′-GACAGCAGTCAGGATCTGGGAGCAGGAG-3′

Mut 1

Forward

5′-GGAGTATTCGCGCTCACTTGCCTGCCTCTGTCT-3′

Reverse

5′-TGAGCGCGAATACTCCACAAAGTCCACCTGG-3′

Mut 2

Forward

5′-GCTGGGCCTTTCTCCCACACCCCCTCCTACCCT-3′

Reverse

5′-GAGAAAGGCCCAGCCCCCGACAGCCAGCGC-3′

Mut 3

Forward

5′-TCTGGAAAGGCCCAGGTCTTCTGCGATCAGTTA-3′

Reverse

5′-CCTGGGCCTTTCCAGATTGAAAGGGGTACAGGA-3′

Mut 4

Forward

5′-CACATGCGAATAAGACGGAGGAAAAAACAAACA-3′

Reverse

5′-TCTTATTCGCATGTGTGTGTGTGCCATGCAC-3′

Mut 5

Forward

5′-ATAGTAAAGGTTGGTATGTATGTGCCTGTGGGT-3′

Reverse

5′-ACCAACCTTTACTATAGCAACAGCGTGTGCAAG-3′

  1. Underscored bases in primer sequences indicate mutations in the putative HIF-1α binding element
  2. Mut, mutant type