Fig. 4

Association between S100A4 expression and vascularization in GBM. A Upper: HE staining and IHC for CD34 in non-Ps and Ps perinecrotic and non-necrotic lesions of GBMs. Note the dense CD34 immunoreactivity (closed circle) in non-Ps perinecrotic lesions, in contrast to the scattered immunopositivity (indicated by arrows) in Ps perinecrotic and non-necrotic lesions. Necrotic areas are indicated by asterisks and partitioned by dotted lines. Original magnification, × 100. Scale bar = 200 μm. Lower: microvascular density (MVD) determined by CD34 immunoreactivity in non-Ps and Ps perinecrotic and non-necrotic lesions. The data are presented as means ± SDs. B Left: staining by HE and IHC for the indicate molecules in adjacent, vascular-rich, and distal areas of non-Ps perinecrotic lesions of GBMs. The three lesions are partitioned by dotted lines. Necrotic areas are indicated by asterisks. Insets show the magnified views of the boxed area. Original magnification, × 100 and × 400 (inset). Scale bar = 200 μm. Right: microvascular density (MVD) determined by CD34 immunoreactivity (upper) and IHC score for the indicated molecules in adjacent (adj), vascular-rich (vas), and distal (dis) areas of non-Ps perinecrotic lesions of GBMs. The data are presented as means ± SDs