Fig. 5

PERK-dependent ER stress activation and apoptosis induction by CPX. A The expression of ER stress and apoptosis related proteins in H1299 and 95D cells with the treatment of CPX (0, 5, 10 and 20 μM) and detected by Western blotting. B H1299 and 95D cells with the treatment of CPX (0 and 20 μM), CPX (20 μM) combined with 10 mM NAC or 10 mM NAC alone for 48 h. Western blotting was used to detect proteins related to ER stress and apoptosis. C H1299 and 95D cells with the treatment of CPX (0 and 20 μM), CPX (20 μM) combined with 10 mM NAC or 10 mM NAC alone for 48 h, and the Intracellular ROS level was determined. D H1299 and 95D cells with the treatment of CPX (0 and 20 μM), CPX (20 μM) combined with 10 mM NAC or 10 mM NAC alone for 48 h, and mitochondrial ROS was determined. E H1299 and 95D cells with the treatment of CPX (0, 5, 10, 20 μM) for 48 h, apoptosis rate was detected. F H1299 and 95D cells with the treatment of CPX (0 and 20 μM), CPX (20 μM) combined with the 10 mM NAC or 10 mM NAC alone for 48 h, apoptosis rate was detected. Data were presented as mean ± SD (n = 3, **for p < 0.01, ***for p < 0.001, ****for p < 0.0001)