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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Ciclopirox targets cellular bioenergetics and activates ER stress to induce apoptosis in non-small cell lung cancer cells

Fig. 1

CPX suppresses the growth of NSCLC cells in vitro. A H1299, 95D, HK2 and 293T cells with the treatment of different concentrations of CPX or vehicle control for 72 h. The relative cell viability for CPX in H1299, 95D, HK2 and 293T cells was determined using CCK-8 assay kits. B Cell viability assay of H1299 and 95D cells following treating with various concentrations of CPX (0, 10, 20 and 40 μM) at 1, 2, 3 and 4 days determined by CCK-8 assay. C and D 1 × 103 H1299 and 95D cells inoculated and cultured in 6-well plates for 5 days, following treating with indicated concentrations of CPX or vehicle control for one week. Representative images showing crystal violet staining of cell colonies (C), and colony forming efficiency was determined (D). E and F The distribution of cell cycle was analyzed by flow cytometry. G H1299 and 95D cells treated with various concentrations of CPX as indicated for 48 h. Expression level of cell cycle-related proteins were detected. Data are presented as mean ± SD (n = 3, ns: no significance for indicated comparison. **for p < 0.01, ***for p < 0.001, ****for p < 0.0001)

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Cell Communication and Signaling

ISSN: 1478-811X