Fig. 4
From: An NF-κB/OVOL2 circuit regulates glucose import and cell survival in non-small cell lung cancer
OVOL2 negatively regulates the NF-κB signaling pathway through its interaction with P65. A NF-κB-specific luciferase assay in OVOL2-overexpressing BEAS-2B cells stimulated with TNFα. BEAS-2B cells were transiently transfected with the indicated amount of plasmids and then NF-κB signaling was stimulated by adding recombinant TNFα protein to culture medium. Western blotting indicated OVOL2 expression. B NF-κB-specific luciferase assay in OVOL2-overexpressing A549 cells. Western blotting indicated OVOL2 expression. C Co-IP assay of HEK293T cells transfected with FLAG-tagged OVOL2 and HA-tagged P65. D Co-IP assay of HEK293T cells transfected with HA-tagged P65 and FLAG-tagged full-length, zinc finger-deleted or SNAG motif-deleted OVOL2. E In vitro pulldown assays of different recombinant proteins between His-tagged P65 and GST-tagged full-length, zinc finger-deleted or SNAG motif-deleted OVOL2. F Endogenous co-IP assay between OVOL2 and P65 in NCC-827 cells. For A and B, the data are expressed as the means ± SDs; n = 3 per group. **, p < 0.01 and ***, p < 0.001