Fig. 3
From: An NF-κB/OVOL2 circuit regulates glucose import and cell survival in non-small cell lung cancer
OVOL2 regulates aerobic glycolysis in NSCLC cells through the NF-κB/GLUT1 pathway. A Western blot analysis of GLUT proteins in OVOL2-overexpressing A549 and SK-MES-1 cells. B Western blot analysis of the GLUT1 protein in OVOL2-knockdown NCI-H1299 and NCI-H661 cells. C Cellular glucose uptake and lactate release levels were measured in OVOL2-knockdown NCI-H1299 cells after knockdown of membrane GLUT1 to the control level. D Western blot analysis of the GLUT1 protein in OVOL2-overexpressing A549 cells after overexpression of P65. E Assessment of GLUT1 localization by IF staining in the cells described in D. F Cellular glucose uptake and lactate release levels were measured in the cells described in D. For C and F, the data are expressed as the means ± SDs; n = 3 per group. *, p < 0.05; **, p < 0.01. Scale bar: 50 mm