The granulosa cell response to luteinizing hormone is partly mediated by YAP1-dependent induction of amphiregulin

Table 1 List of genes downregulated following loss of Yap1/Taz whose expression is LH and/or Erk1/2-dependent

Gene Symbol	FC in Yap1/Taz depleted GCs	FC in LH-stimulated GCs	FC in Erk1/2 depleted GCs
Abi3bp	− 1.60		− 1.91
Arhgap32	− 1.51	1.62
Arid5b	− 1.90		− 1.56
Arrdc3	− 1.88	2.94
Bpgm	− 1.57	1.61
Bzw1	− 1.72	1.51
Cblb	− 1.59		− 2.46
Ccnl1	− 1.56	1.88
Ccno	− 1.76	4.41
Cited2	− 2.00		− 2.00
Cpne3	− 1.52		− 1.96
Efnb2	− 1.63	1.58	− 4.58
Egr1	− 1.62		− 37.71
Emb	− 1.53		− 3.67
Emp1	− 1.69		− 5.60
Ereg	− 1.55	7.70	− 2.09
Errfi1	− 2.25		− 6.00
Fam107b	− 1.95	1.77
Far1	− 1.75	1.67
Fbxw14	− 1.70	2.95
Fbxw15	− 1.62	3.51
Fbxw19	− 1.86	2.89
Fbxw20	− 2.14	3.08
Fbxw21	− 2.88	2.40
Fbxw22	− 1.64	2.26
Ggct	− 1.56	1.55
Glrx	− 1.92	1.54
Gng3	− 1.94	1.64
Hccs	− 1.93		− 1.61
Hsd3b1	− 1.81	1.54
Igf2bp2	− 1.83	1.69
Il23a	− 1.59		− 2.67
Inhba	− 2.02	3.64
Jag1	− 2.03	1.62	− 1.75
Kdr	− 1.94	1.69
Khdc1a	− 1.63	1.69
Lrrc8c	− 1.54		− 1.86
Mid1	− 1.58	1.87	− 3.12
Nfya	− 1.97	1.52
Nlrp14	− 2.09	2.69
Nlrp2	− 1.53	1.87
Nlrp4a	− 2.31	1.74
Oas1c	− 1.64	2.78
Oas1e	− 2.15	2.80
Osgin2	− 1.96	3.23	− 1.69
Phldb2	− 1.61		− 4.09
Pik3r1	− 1.73		− 2.63
Plat	− 1.80	1.56
Plau	− 1.54	2.03	− 10.36
Plk2	− 1.59	3.71	− 2.47
Prkg2	− 1.59		− 2.64
Ptgs2	− 3.06	1.98	− 42.47
Ptp4a1	− 1.51	2.42
Rbm47	− 1.96	1.54	− 3.11
Rgs2	− 2.13	3.05
Rnd3	− 1.72	1.51	− 12.90
Rsbn1	− 1.78	1.76
Rspo2	− 1.55	1.88
Serpinb2	− 1.66		− 4.39
Sfrp4	− 1.99	1.93	− 6.40
Siah1b	− 1.54	1.64
Slc7a8	− 2.36	2.09	− 1.69
Spin1	− 2.81	1.56
Tcl1b1	− 1.93	1.91
Tiparp	− 2.00	1.66	− 1.63
Tnfaip6	− 2.27	6.65	− 10.01
Zfp804a	− 1.68		− 3.50
Znrf2	− 1.63		− 2.03

Genes listed are downregulated following 18 h of Yap1/Taz knockdown in GCs, and meet one of the two following criteria: (1) upregulated in WT GCs stimulated 3 h with LH, or (2) downregulated in Erk1/2-deficient GCs isolated 2.5 h after hCG stimulation. Genes are listed in alphabetical order. Fold change (FC) is expressed as a ratio between the treatment (Ad-Cre, LH, Erk1/2 depleted GCs) and its respective control (Ad-eGFP, PBS, WT GCs). Oocyte-specific genes were excluded from this list as their presence was likely caused by oocyte contamination of our primary GC culture. Cut-off thresholds of P ≤ 0.05 and |FC|≥ 1.5 were used to identify differentially expressed genes

ISSN: 1478-811X