Fig. 5
Loss of Yap1/Taz expression in murine granulosa cells negatively affects the expression of several ovulation-related genes. Granulosa cells isolated from Yap1f/f;Tazf/f mice were infected with adenoviruses expressing either Cre recombinase (Ad-Cre; to knockdown Yap1 and Taz expression) or eGFP (Ad-eGFP; control) for 18 h. A microarray analysis was conducted to identify differentially expressed genes (DEGs). A Pie chart representing the Panther gene-ontology (GO) functional classification of the DEGs following Yap1 and Taz knockdown. B Venn Diagram highlighting the overlap between DEGs following loss of Yap1/Taz expression vs LH stimulation for 3 h in primary murine GCs. C Metascape enrichment network visualization showing clustering of the Hippo and LH-dependent genes according to their GO biological processes. Cluster annotations are shown in color code next to the bar graph representing statistical significance (only P < 0.01 are shown) of the said enriched biological processes. D Venn Diagram highlighting the overlap between DEGs following loss of Yap1/Taz expression vs Erk1/2-deficient GCs treated with hCG for 2.5 h