Fig. 4

Loss of Yap1 and Taz blunts LH responsiveness. A, B Primary GCs isolated from Yap1^f/f;Taz^f/f mouse ovaries were infected with adenoviruses expressing either Cre recombinase (Ad-Cre; to knockdown Yap1 and Taz expression) or eGFP (Ad-eGFP; control) for 18 h. GCs were treated with either LH (A), FSK (B) or PBS (control) for 2 h (n = 4 replicates/treatment, done three times). Data were normalized to the housekeeping gene Rpl19. C) Primary Yap1^f/f;Taz^f/f GCs were infected with Ad-Cre for 24 h (Ad-eGFP for control). GCs were treated with LH (PBS for control) for 30 min. Representative immunoblots show 2 replicates per condition, whereas quantification was done using 12 replicates per condition. β-actin (ACTB) was used as the loading control. Data were all normalized by the sum of all data points in a replicate and were subjected to arcsine transformation, as they are expressed as a proportion (0–1) of the whole replicate. Different letters above histograms indicate significant differences between treatment conditions. Two-way ANOVA (Tukey’s post hoc test): P ≤ 0.05