Fig. 3

LH acts via PKA to phosphorylate YAP1. Primary cultured GCs were pretreated with or without inhibitors against PKA (H-89 or PKI) followed by treatment with LH or vehicle control for 30 min. Representative immunoblots show 1 replicate per condition, whereas quantification was done using 4 replicates per condition. β-actin (ACTB) was used as the loading control. Data were normalized by the sum of all data points in a replicate and were subjected to arcsine transformation, as they are expressed as a proportion (0–1) of the whole replicate. Different letters above histograms indicate significant differences between treatment conditions. Two-way ANOVA (Tukey’s post hoc test): P ≤ 0.05