Fig. 2

LH activates the Hippo pathway in granulosa cells in vitro. Primary GCs were treated with or without LH on a time course and the expression of the indicated proteins was evaluated by western blotting. Representative immunoblots show 2 replicates per time point, whereas quantification was done using 4 replicates per time point. β-actin (ACTB) was used as the loading control. Data were normalized by the sum of all data points in a replicate and were subjected to arcsine transformation, as they are expressed as a proportion (0–1) of the whole replicate. Data were compared to 0 min LH. One-way ANOVA (Dunnet’s post hoc test): *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001