Fig. 5

E2 ameliorated EF% and Ca²⁺ transient by increasing SERCA2a expression. a M-mode of echocardiography of each group. b EF% of the rats in each group (n = 4; **P < 0.01, ***P < 0.001 vs. sham; ^##P < 0.01, ^###P < 0.001 vs. OVX; ^$$P < 0.01, ^$$$P < 0.001 vs. OVX + E2; ^%P < 0.05 vs. I/R; ^&P < 0.05, ^&&P < 0.01 vs. OVX + I/R; ^@P < 0.05 vs. OVX + E2 + I/R; ^{^}P < 0.01 vs. Ad-EGFP + I/R). c Wave of the ratio 340/380 nm in Ca²⁺ transient of each group in vitro. d–f The trend of the resting intracellular Ca²⁺ levels (F0), maximal value of intracellular Ca²⁺ transient (Peak), and the diastolic intracellular Ca²⁺ transient decay time constant (Tau) (n = 5; *P < 0.05, **P < 0.01, ***P < 0.001 vs. con; ^#P < 0.05, ^##P < 0.01 vs. H/R; ^$P < 0.05, ^$$P < 0.01 vs. E2 + H/R; ^%P < 0.05, ^%%P < 0.01 vs. Ad-EGFP + H/R; ^&P < 0.05, ^&&P < 0.01 vs. Ad-SERCA2a + H/R; ^@P < 0.05, ^@@P < 0.01 vs. si-NC + H/R; ^{^^^}P < 0.001 vs. si-SERCA2a + H/R; ^aaaP < 0.001 vs. Ad-SERCA2a + E2 + H/R)