Fig. 6

Disruption of transcriptional process, but not CDK7 inhibition, was required for nutlin-3 + THZ1 mediated apoptosis. A Western blot was utilized to verify the silencing effects of CDK7 shRNA plasmids in MCF-7 cell. B Quantity of CDK7 in A. C CCK8 assay was utilized to measure the viability of MCF-7 cell with CDK7 shRNA at 24 h, 48 h and 72 h. D Apoptosis was analyzed by flow cytometry after MCF-7 cell was treated with CDK7 shRNA and control shRNA. E Quantity of apoptosis in D. F CCK8 assay was used to detect the cell viability of MCF-7 cell with control shRNA or CDK7 shRNA under increasing concentrations of nutlin-3 treatment for 24 h or 48 h separately. G CCK8 assay was used to detect the cell viability of MCF-7 cell under increasing concentrations of flavopiridol treatment combining 20uM nutlin0-3 for 24 h or 48 h separately. H CCK8 assay was used to detect the cell viability of MCF-7 cell under increasing concentrations of THZ1 treatment combining 20uM nutlin0-3 for 24 h or 48 h separately. I CCK8 assay was used to detect the cell viability of MCF-7 cell under increasing concentrations of LDC4297 treatment combining 20uM nutlin0-3 for 24 h or 48 h separately. Statistically significant (P < 0.05) are *, (P < 0.01) are **