Table 1 Primer sequences for RT-qPCR
Gene | Primer sequence (5′–3′) |
|---|---|
Cyclin D2 | F: GCAGAAGGACATCCAACCGTAC |
R: ACTCCAGCCAAGAAACGGTCCA | |
PCNA | F: AGTTTTCTGCGAGTGGGGAG |
R: AAGACCTCAGAACACGCTGG | |
Bax | F: CACGTCTGCGGGGAGTCA |
R: TAGGAAAGGAGGCCATCCCA | |
Fibronectin-1 | F: GGATCCCCTCCCAGAGAAGT |
R: GGGTGTGGAAGGGTAACCAG | |
Collagen IV | F: CGGGTGTGAAAAGACCTATCGG |
R: CTGGCATTCCTCTGACGCCTTT | |
PUMA | F: ACCGCTCCACCTGCCGTCAC |
R: ACGGGCGACTCTAAGTGCTGC | |
PSMD14 | F: GTCAGTGTGGAGGCAGTTGATC |
R: CCACACCAGAAAGCCAACAACC | |
TGFBR1 | F: TGCTCCAAACCACAGAGTAGGC |
R: CCCAGAACACTAAGCCCATTGC | |
GAPDH | F: GTCTCCTCTGACTTCAACAGCG |
R: ACCACCCTGTTGCTGTAGCCAA | |
miR-212-3p | F: ACCTTGGCTCTAGACTGCTT |
R: GAACATGTCTGCGTATCTC | |
miR-219a | F: TGATTGTCCAAACGCAATTC |
R: GAACATGTCTGCGTATCTC | |
miR-455 | F: TGTGCCTTTGGACTACATC |
R: GAACATGTCTGCGTATCTC | |
miR-483-5p | F: ACGGGAGAAGAGAAGGGA |
R: GAACATGTCTGCGTATCTC | |
miR-511-3p | F: TGCCTTTTGCTCTGCACTC |
R: GAACATGTCTGCGTATCTC | |
miR-378-5p | F: CTGACTCCAGGTCCTGTG |
R: GAACATGTCTGCGTATCTC | |
miR-202-3p | F: TTCCTATGCATATACTTCT |
R: GAACATGTCTGCGTATCTC | |
U6 | F: CTCGCTTCGGCAGCACAT |
R: TTTGCGTGTCATCCTTGCG |