Fig. 7

Silencing of PSMD14 blocks PDGF-induced abnormal proliferation of rMCs. A, B mRNA (A) and protein expression of PSMD14 in rMCs after shPSMD14 transfection determined by RT-qPCR and western blot analysis, respectively; C determination of the ubiquitination level of TGFBR1; D expression of TGF-β1 and phosphorylation of Smad2/3 in rMCs determined by western blot analysis; E proliferation of rMCs determined by EdU labeling; F, viability of cells determined using a CTG kit; G portion of dead cells detected by EB fluorescence staining; H apoptosis of cells determined by Hoechst 33,258 staining; I, apoptosis rate of cells determined by flow cytometry; J protein expression of Cyclin D2, PCNA, Bax and PUMA in cells determined by western blot analysis. Data were expressed as the mean ± SD from at least three independent experiments. Data were analyzed by one-way ANOVA followed by Tukey’s multiple comparison test. **p < 0.01 versus Scr group