Fig. 6

PSMD14 promotes TGFBR1 stability through deubiquitination modification. A, B mRNA (A) and protein (B) expression in rat renal tissues determined by RT-qPCR and western blot analysis, respectively; C, D mRNA (C) and protein (D) expression in rMCs determined by RT-qPCR and western blot analysis, respectively; E, F binding relationship between PSMD14 and TGFBR1 validated through a Co-IP assay; G ubiquitination level of TGFBR1 in 293 T cells after overexpressing Flag-TGFBR1, HA-Ub and PSMD14 in cells. Data were expressed as mean ± SD from at least three independent experiments. Data were analyzed by one- or two-way ANOVA followed by Tukey’s multiple comparison test. **p < 0.01, ***p < 0.001 versus or PBS group; ##p < 0.01 versus MsPGN + PBS group or 0 μg/ml group; @@ p < 0.01 versus Evs/Inhibitor NC group; n.s. represents no significance