Fig. 4

ucMSC-Evs carry miR-378 to negatively regulate PSMD14. A a heatmap for the top 30 DE miRNAs in rat renal tissues and rMCs before and after 40 μg/mL Evs treatment according to a miRNA microarray analysis; B selection of the shared DE miRNAs in tissues and cells; C-D, expression of 7 upregulated miRNAs in renal tissues (C) and rMCs (D) after ucMSC-Evs treatment determined by RT-qPCR; E a functional classification analysis among the target mRNAs of miR-378 performed on the Panther System; F–G mRNA (F) and protein (G) expression of PSMD14 in rat renal tissues determined by RT-qPCR and western blot analysis, respectively; H, I mRNA (H) and protein (I) expression of PSMD14 in rMCs determined by RT-qPCR and western blot analysis, respectively; J binding relationship between miR-378 and PSMD14 determined using the luciferase reporter gene assay. In animal models, n = 6 in each group. Data were expressed as mean ± SD from at least three independent experiments. Data were analyzed by one- or two-way ANOVA followed by Tukey’s multiple comparison test. **p < 0.01 versus sham group or PBS group; ##p < 0.01versus PDGF-BB group or 0 μg/mL group; @@ p < 0.01 versus mimic control