Fig. 3

Combination of WNT974 and ART induces KRAS protein degradation by increasing anaphase promoting complex subunit 2 (ANAPC2) expression. A HCT116 cells were treated with 20 μM WNT974, 20 μM ART or the combination of both for 48 h. Heat map showing the PCR array results. B The numbers of up- or down-regulated genes in the PCR array. C The mRNA expressions of CUL7 (cullin), ANAPC2 (anaphase promoting complex subunit 2), UBE2M (ubiquitin conjugating enzyme E2M), SYVN1 (synoviolin 1) and RNF123 (RING finger protein123) in the CRC cells after the treatments. KRAS expression in the CRC cells after siRNA-mediated knockdown of D CUL7, E ANAPC2, F UBE2M, G RNF123, H SYVN1, upper panel showing the expressions of CUL7, ANAPC2, UBE2M, RNF123 and SYNV1 in these cells. I Protein expressions of ANAPC2 in ANAPC2-overexpressed CRC cells. J Protein expressions of KRAS in ANAPC2-overexpressed CRC cells in the presence or absence of MG132. K CRC cells were treated with 20 μM WNT974, 20 μM ART or the combination of both for 48 h. The protein expression of ANAPC2 after the treatments. Shown is mean ± SE, n = 3 individual experiments, *p < 0.05, *** < 0.001 compared to control; a < 0.05, compared to ART; b < 0.05, compared to WNT974