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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Combination of artesunate and WNT974 induces KRAS protein degradation by upregulating E3 ligase ANACP2 and β-TrCP in the ubiquitin–proteasome pathway

Fig. 1

Combination of WNT974 and artesunate (ART) reduces KRAS protein level and activity in CRC cells. A Fraction affected (Fa) versus combination index (CI) plots were used to determine the extent of synergy for combination treatment in 8 CRC cells, synergistic effects are defined as CI < 1. B HCT116 and SW620 were treated with 20 μM WNT974, 20 μM ART or the combination of both for 48 h. The NRAS, HRAS KRAS protein level and KRAS activity were examined. C KRAS mRNA level in HCT116 cells and SW620 cells after treatments. Shown is mean ± SE, n = 3 individual experiments, *p < 0.05, compared to control; a < 0.05, compared to ART; b < 0.05, compared to WNT974

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Cell Communication and Signaling

ISSN: 1478-811X