Fig. 8

METTL14 depletion enhances ccRCC cell metastasis, EMT and PI3K/AKT signal by overexpressing ITGB4. a, b Protein levels of ITGB4, N-cadherin, E-cadherin, p-PI3K, PI3K, p-AKT and AKT in ACHN cells (a) and Caki-1 cells (b) with indicated treatments detected by western blot. c, d Migration assay (c) and invasion assay (d) used for detecting the migratory abilities and invasiveness of cells with indicated treatments, respectively. Bar scale = 50 μm. e Representative and HE-staining images of lungs excised from mice injected with cells of indicated disposals. Bar scale = 50 μm. Each experiment was performed independently for three times. Bar graphs: means ± SDs. *p < 0.05, **p < 0.01, ***p < 0.001. ns, not significant