Fig. 3

ITGB4 promotes migration, invasion, metastasis and EMT process of ccRCC cells. a Overexpression of ITGB4 in ACHN and Caki-1 cells respectively confirmed by western blot. b Wound-healing assay used to detect the alteration of cell migratory ability after ITGB4 overexpression. Bar scale = 200 μm. c, d Cell migration and cell invasiveness respectively detected by migration assay (c) and invasion assay (d). e Expression of EMT-related markers in ACHN and Caki-1 cells with or without ITGB4 overexpression determined by western blot. f Representative and HE-staining images of lungs excised from mice injected with ITGB4-overexpressing and negative control cells. Bar scale = 50 μm. Each experiment was carried out independently for three times. Bar graphs: means ± SDs. *p < 0.05, **p < 0.01 and ***p < 0.001