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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: METTL14-mediated N6-methyladenosine modification of ITGB4 mRNA inhibits metastasis of clear cell renal cell carcinoma

Fig. 2

ITGB4 depletion inhibits migration, invasion and EMT process of ccRCC cells. a ITGB4 protein level in 5 ccRCC cell lines confirmed by western blot. b The knockdown efficiencies of the siRNAs in ACHN cells and Caki-1 cells respectively proved by western blot assay. c Wound-healing assay used to detect cell migration. Bar scale = 200 μm. d, e Cell migration and cell invasiveness respectively detected by migration assay (d) and invasion assay (e). Bar scale = 50 μm. f Expression of EMT-related markers, N-cadherin and E-cadherin, in ACHN and Caki-1 cells with indicated treatments determined by western blot. An independent triplicate was performed for each experiment. Bar graphs: means ± SDs. *p < 0.05; **P < 0.01 and ***p < 0.001

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Cell Communication and Signaling

ISSN: 1478-811X