Fig. 1

Overexpression of ITGB4 is associated with occurrence and poor prognosis of ccRCC. a, b The abundance of ITGB4 transcripts in ccRCC tissues and normal kidney tissues from TCGA database (a) and GSE53757 dataset (b). c, d Kaplan–Meier analysis of ccRCC patients’ overall survival (c) and disease-free survival (d) based on ITGB4 expression in TCGA database. e–h ITGB4 expression of ITGB4 in ccRCC tissues of different clinical stages (e), pathological grades (f), nodal metastasis status (g) and subtypes (h). i Analysis of ITGB4 protein level in ccRCC tissues and normal kidney tissues in CPTAC database. j The mRNA expression of ITGB4 in 50 paired ccRCC tissues and normal kidney tissues detected by qRT-PCR. k The protein expression of ITGB4 in 24 ccRCC paired tissues and normal kidney tissues determined by western blot assay. N, normal tissues; T, tumor tissue. l ITGB4 expression in metastatic ccRCC and non-metastasis ccTCC detected by immunohistochemistry assay. Bar scale = 20 μm. Bar graphs: means ± SDs. *p < 0.05; ****p < 0.0001