Table 3 Primer sequences for real-time PCR
From: Energy deprivation-induced AMPK activation inhibits milk synthesis by targeting PrlR and PGC-1α
Genes | Forward primers | Reverse primers | Size, bp |
|---|---|---|---|
FASN | AGCACTGCCTTCGGTTCAGTC | AAGAGCTGTGGAGGCCACTTG | 94 |
ACACA | GAAGTCAGAGCCACGGCACA | GGCAATCTCAGTTCAAGCCAGTC | 119 |
FABP3 | ACCTGGAAGCTAGTGGACAG | TGATGGTAGTAGGCTTGGTCAT | 106 |
DGAT1 | CAGCTGTGGCCTTACTGGTTGA | CGGCACCACAGGTTGACATC | 118 |
SREBP1 | AGAAGCTCAAGCAGGAGAACCTGA | ACTTCGGGTTTCATGCCCTCCATA | 127 |
AMPK | AACCTGAGAACGTCCTGCTTGATG | TGACTTCTGGTGCGGCATAATTGG | 132 |
CSNS1 | AATTCTTCCAGCTTGGTGCCTCTC | CAATGCCTTCAGGGGTGTCGTAG | 105 |
CSN2 | AGGAACAGCAGCAAACAGAGGATG | GGCTGAAGAGGCGGCAGAAAG | 136 |
WAP | ACAGAGTGTATCATCTGCCAAA | ACCAATGTTGACAGGAGTTTTG | 102 |
PCG-1α | CAGAGAGTATGAGAAGCGAGAG | AGCATCACAGGTATAACGGTAG | 232 |
PrlR | CCTGAAATCCACAAATGTCGTT | CATATGGAAGTGTACTGCTTGC | 197 |
PIP | CATCAACAGCACAGGAAAATCA | AGGATGATCATTACAGAGGCAG | 158 |
BCAT | TTTGCTGGAGAAAGAGGATCAG | GCTTCTCTTTTAAGACAGTGGC | 91 |
GPT | CTTCAAGCAGTTTCAAGCAGAG | TTGAGGGAAGGAATACATAGCG | 139 |
GOT | ACAAGAACACACCAATCTACGT | ATAGGGCCGAATGTCCTTAAAA | 92 |
ASCT2 | TCCTTTCTAGATCTCGTGAGGA | CTGGACCATGGTTGAATTGATC | 150 |
ASCT1 | GCAGGACAGATTTTCACCATTC | CAAGATGATGGCAATTGTGAGG | 99 |
xCT | CTATTTTACCACCATCAGTGCG | ATCGGGACTGCTAATGAGAATT | 102 |
CAT2 | CATCGGAATAGTGACGTCCTTA | AAGGCATCATAAGCGTTAAAGC | 80 |
CAT1 | CTGTGTTTTGGTCTTACGGTAC | AAAAAGCCTGTCTGTGATTCAC | 132 |
4F2hc | AGGGACTCCTGTTTTTAGCTAC | GTGAAAGATGCTGGACTCATTC | 109 |
LAT2 | TGGATCCTTACAAGAACCTTCC | CATTGCAGTGACGTATGCAATA | 98 |
ACTIN | CCACCATGTACCCAGGCATT | CGGACTCATCGTACTCCTGC | 190 |