Fig. 5
From: Energy deprivation-induced AMPK activation inhibits milk synthesis by targeting PrlR and PGC-1α
Knock-down of PrlR lowers synthesis of milk protein but little affects milk fat. A and B TAG concentration in cell (A) and medium (B), n = 6. C Confocal microscopy of HC11 immunostained for α-casein (green), β-casein (green), WAP (green) and DAPI (blue). Scale bars, 100 μm. The small red boxes in the figure are typical areas selected, and the three small images on the right are the enlarged images of the target protein, nucleus and merge images in selected areas, respectively. D and E Western blots of HC11 lysates for critical milk protein synthesis genes. D Representative western-blotting bands hybridized with target proteins including α-casein, β-casein, WAP and Actin antibodies, n = 3. E Result of analyzing the WB bands. In this figure, cells (transfected by siRNA in advance) for TAG determination, immunofluorescence staining and WB were collected after 24 h incubation with 10 mM glucose medium and (10 mM glucose + 300 μM Phe) medium. All datas with error bars are averages ± SEM. In histograms, the numbers above the column are the p-values marked as a range if significantly different (P < 0.05) and each small black dot represents a test value