Fig. 4
From: Energy deprivation-induced AMPK activation inhibits milk synthesis by targeting PrlR and PGC-1α
Activated AMPK induces the downstream PrlR/JAK2/STAT5/PIP pathway. A PCA of RNA-seq, n = 3. B GSEA showing enrichment of prolactin receptor binding in HC11 differentially expressed genes. The graph (left) shows the result of the control group-vs-glucose group and another graph (right) shows glucose group-vs-PHE group. C and D Volcano plots based on the significantly different genes in each comparison group. C Volcano plot of the control group-vs-glucose group and D Glucose group-vs-PHE group. The dots that the arrows point to are PIP. E and F (E) are top 10 significantly up-regulated 10 genes in the con-vs-glu comparison, and (F) are top 10 significantly down-regulated in the glu-vs-PHE comparison. G Relative mRNA expressions of PrlP and PIP, n = 3. H and I Western blots of HC11 lysates for PrlR/JAK2/STAT5 pathway. In (H), bands were incubated with target proteins and Actin antibodies, n = 3. Protein expressions of target proteins are shown in (I), n = 3. In this figure, cells for WB were collected after 24 h incubation with 0 mM glucose medium, 10 mM glucose medium and (10 mM glucose + 300 μM Phe) medium and cells for real-time PCR and RNA-seq are 12 h. All data with error bars are averages ± SEM. In histograms, the numbers above the column are the p-values marked as a range if significantly different (P < 0.05) and each small black dot represents a test value