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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: Endothelial colony-forming cell-derived exosomal miR-21-5p regulates autophagic flux to promote vascular endothelial repair by inhibiting SIPL1A2 in atherosclerosis

Fig. 6

ECFC-derived exosomes rescue autophagic flux in ox-LDL-treated HMECs through miR-21-5p. A Expression of miR-21-5p in ECFC exosomes transfected with negative control inhibitor or miR-21-5p inhibitor was detected by qRT–PCR. Biological replicates = 3, and technical replicates = 3. B The expression of autophagy-related proteins (LC3I, LC3II, and p62) in HMECs with different treatments was detected by western blot assay. Biological replicates = 3, and technical replicates = 1. C Representative images of HMECs transfected with Ad-mCherry-GFP-LC3B adenovirus followed by different treatments. Scale bars = 100 μm. Exos-miR inh ECFC-exosomes were transfected with miR-21-5p inhibitor, Exos-NC inh ECFC-exosomes were transfected with NC inhibitor. Biological replicates = 3 and technical replicates = 1. Significant differences between different treatment groups are indicated as *P < 0.05

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Cell Communication and Signaling

ISSN: 1478-811X