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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Endothelial colony-forming cell-derived exosomal miR-21-5p regulates autophagic flux to promote vascular endothelial repair by inhibiting SIPL1A2 in atherosclerosis

Fig. 1

Characterization of human ECFCs. A Morphology of EPCs cultured for 0, 3, 7, 10 and 21 days. Scale bar = 100 μm. B Immunofluorescence staining of endothelial progenitor cell-specific surface markers (CD144, eNOS, vWF and CD34) and haematopoietic cell-specific markers (CD45) in the cells. Nuclei were counterstained using DAPI. Scale bar = 100 μm. C Flow cytometry analysis showing that EPCs were positive for CD144, CD34 and vWF but negative for the haematopoietic cell-specific marker CD45. D Immunofluorescence examination of ac-LDL (Dil) uptake and UEA-1 (FITC) binding capability of ECFCs. Nuclei were counterstained with DAPI. Scale bar = 50 μm. E The morphology of ECFC-derived exosomes was examined by TEM. Scale bar = 100 nm. F The surface markers CD63, CD9, and CD81 of exosomes were examined by western blot assay. Exos ECFCs-exosomes. G The particle sizes and concentrations of ECFC-exosomes were measured using NanoSight. Biological replicates = 3, and technical replicates = 1

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Cell Communication and Signaling

ISSN: 1478-811X