Fig. 6
From: Sec23a inhibits the self-renewal of melanoma cancer stem cells via inactivation of ER-phagy
FAM134B promotes the stemness of M14-SE cells and Sec23a is a favorable diagnostic and prognostic marker for SKCM. a Analysis of mRNA expression of Aldh1, CD133, Nanog, Oct4 and Sox2 in M14-SE-shSec23a cells with FAM134B interference. b–d Clonogenic spheroid formation rate, morphology and average spheroid diameter of M14-SE-shSec23a cells with FAM134B interference in the single-cell cloning assay. e Serial spheroid formation assay of M14-SE-shSec23a cells with FAM134B interference, bar = 200 µm. f. Tumors resected from nude mice inoculated with M14-SE-shSec23a cells with FAM134B interference, bar = 1 cm. g and h Tumor volume and weight. i Low Sec23a expression levels indicated poor overall survivals for SKCM patients using Kaplan–Meier Plots analysis. j and k Correlation between Sec23a expression and tumor stages in SKCM patients using the TCGA database. *P < 0.05, **P < 0.01, ***P < 0.001