Fig. 6From: Glycogen synthase kinase GSK3α promotes tumorigenesis by activating HIF1/VEGFA signaling pathway in NSCLC tumorGSK3αregulated tumor angiogenesis in a HIF1α-dependent manner in vitro. a, b Construction of NSCLC cell lines stably expressing vector + shnc, GSK3α + shnc, GSK3α + shHIF1α#1, and GSK3α + shHIF1α#2 in hypoxia. Successful transfection was confirmed via Western blot (a), and ELISA (b) analyses; *p < 0.05. c, d, g HUVECs cultured in different conditional media under hypoxia, followed by transwell assays (c), CCK8 assays (d), and tube formation assays (g); *p < 0.05, **p < 0.05. e, f HUVECs cultured in different conditional media under normoxia, and analyzed by transwell assays (e), and tube formation assays (f); **p < 0.05Back to article page