Fig. 5

GSK3αregulates HIF1α stabilization in an PHDs-pVHL complex independent way. a Immunoprecipitation and Western blot results showing levels of protein expression in A549 and H226 cells. b Immunoprecipitation and Western blot results showing levels of proteins expression in A549 and H226 cells under hypoxia conditions. MG132 was used to inhibit endogenous protein degradation. c, e Western blots showing expression of proteins from identified cells under hypoxia or DMOG conditions. d Silencing GSK3A expression using lentivirus in A549 cells, MG132 was used to inhibit endogenous protein degradation, followed by Western blot analysis. h Immunoprecipitation and Western blot results showing protein expression in A549 cells transfected with His-ubiquitin plasmid and stably expressing Vector or GSK3α. MG132 was used to inhibit endogenous protein degradation. g Immunoprecipitation and Western blot results showing protein expression in A549 cells transfected with His-ubiquitin plasmid and stably expressing shnc or shGSK3α. MG132 was used to inhibit endogenous protein degradation