Fig. 3

CPNE1 activated the MET signaling pathway in NSCLC. A, B Western blot analysis of RACK1, p-MET, MET, p-AKT, AKT, p-Erk, and Erk protein levels in CPNE1-overexpressing cells compared to control cells, with quantification on the right. C, D Western blot analysis of relevant protein levels in CPNE1 knockdown cells compared to control cells, with quantification on the right. E A human RTK phosphorylation array analysis demonstrated that the p-MET level is increased in CPNE1-overexpressing cells. F Western blot analysis of p-MET in CPNE1 knockdown cell lines treated with HGF (20 ng/mL) for 24 h. *P < 0.05; **P < 0.01; ***P < 0.001