Table 1 Primers designed with existing studies and PrimerBLAST, manufactured by Sigma Aldrich
Gene | Forward primer | Reverse primer |
|---|---|---|
HPRT1 | 5′-TGACACTGGCAAAACAATGCA-3′ | 5′-GGTCCTTTTCACCAGCAAGCT-3′ |
COL1A1 | 5′-ATGCCTGGTGAACGTGGT-3′ | 5′-AGGAGAGCCATCAGCACCT-3′ |
COL2A1 | 5′-AACCAGATTGAGAGCATCCGC-3′ | 5′-CGATAACAGTCTTGCCCCACTTAC-3′ |
COL10A1 | 5′-CACCTTCTGCACTGCTCATC-3′ | 5′-GGCAGCATATTCTCAGATGGA-3′ |
L-SOX5 | 5′-GAATGTGATGGGACTGCTTATGTAGA-3′ | 5′-GCATTTATTTGTACAGGCCCTACAA-3′ |
SOX6 | 5′-CACCAGATATCGACAGAGTGGTCTT-3′ | 5′-CAGGGTTAAAGGCAAAGGGATAA-3′ |
SOX9 | 5′-GCAGGCGGAGGCAGAGGAG-3′ | 5′-GGAGGAGGAGTGTGGCGAGTC-3′ |
ACAN | 5′-AGGGCGAGTGGAATGATGTT-3′ | 5′-GGTGGCTGTGCCCTTTTTAC-3′ |
HSPG2 | 5′-TCAGTCCCTTGTCACCATCCA-3′ | 5′-TAAGCTGCCTCCACGCTTAT-3′ |
COMP | 5′-AACAGTGCCCAGGAGGAC-3′ | 5′-TTGTCTACCACCTTGTCTGC-3′ |