Fig. 2

NCX1 and NCX3 expression and function in rat primary motor neurons. A Immunolocalization of NCX1 and NCX3 isoforms in two different motor neurons within the same culture. B Superimposed representative traces of the effect of Na⁺-free on [Ca²⁺]_i in motor neurons singly transfected with siControl, siNCX1 or siNCX3 (both at 10 nmol/L for 48 h). For details, please refer to Material and Methods. C Quantification of B expressed as Δ% of increase. All the experiments were repeated at least three times; *p < 0.001 versus internal control (basal values of [Ca²⁺]_i), **p < 0.05 versus All. D Quantification of basal values of [Ca²⁺]_i of the treatments of B. *p < 0.05 versus All. E Immunolocalization of NCX1 and SOD1 in motor neuron-enriched culture at two different magnifications