Fig. 8

MyD88S stalls MyD88L overexpression associated myddosome SMOC formation. A chMyD88L overexpression associated formation of myddosome SMOC (granular distribution—refer to Fig. 6C) is hindered by simultaneous overexpression of GFP-MyD88S. Labeled MyD88 variants were overexpressed in HEK293-I3A cells and imaged by confocal microscopy. B Schematic representation of myddosome nuclei expansion assay. Myddosome nuclei containing HA-MyD88L and either Flag-MyD88L or Flag-MyD88S are isolated by immunoprecipitation and contacted with Flag-chMyD88L. Note that the depicted complex stoichiometries are arbitrary chosen and are not determined in the experiment. C MyD88L/MyD88L, but not MyD88S/MyD88L myddosome nuclei are capable of growth by incorporation of additional MyD88L molecules—Western Blot analysis of experiment schematically explained in panel B