Fig. 5

Hydrogen bond network around Tyr116 facilitates myddosome dependent signaling. A Induction of NF-κB-driven reporter expression in HEK293-I3A cells by overexpression of indicated MyD88 variants. Response was normalized to wild type MyD88L. Values are presented as means ± SD from triplicate samples. Statistical significance was determined by Student’s t-test (*p < 0.05, **p < 0.001). B Transgene expression in experiment shown in panel A was analyzed by Western blot. α-tubulin was used as a loading control. C The hierarchical myddosome assembly (PDB ID 3MOP). Intermolecular hydrogen bond network around Tyr116 mediating the homotypic interactions of adjacent MyD88 protomers are shown in close-ups