Fig. 2

Residues Gln114-Leu118 mediate ID function in MyD88 dependent signaling. A Stretches of consecutive residues (indicated in panel B) in MyD88 ID were exchanged to alanine and the effect of mutant overexpression on NF-κB driven reporter expression was evaluated in HEK293-I3A cells. Response was normalized to MyD88L. Values are presented as means ± SD from triplicates. Statistical significance was determined by Student’s t-test (*p < 0.05, **p < 0.001). B Conservation of MyD88 ID across species and residues selected for alanine substitution. C Level of transgene expression in experiment shown in (A) determined by Western blot. α-tubulin was used as the loading control