Fig. 4

Effect of CXCL12 CXCR4 siRNA and co-cultured with fibroblasts on invasiveness of colorectal cancer cells. Colorectal cancer cells were treated with different concentration of CXCL12 or co-cultured with fibroblasts and incubated for 24 h, and then cell invasion were performed by Matrigel assay. Statistical significance was tested by one-way analysis of variance and post hoc test (Turkey Kramer multiple comparisons). All data are expressed as mean ± s.d, *P < 0.01compared with control (A). After transfected with CXCR4 siRNA or co-cultured with fibroblasts colorectal cancer cells were treated with different concentration of CXCL12 and incubated for 24 h, and then cell invasion were performed by Matrigel assay. Statistical significance was tested by one-way analysis of variance and post hoc test (Turkey Kramer multiple comparisons). Statistical significance was indicated by P < 0.05. All data are expressed as mean ± s.d, *P < 0.01compared with control siRNA group (B)