Fig. 4
From: Cytoplasmic eIF6 promotes OSCC malignant behavior through AKT pathway
eIF6 effect is related to the AKT signaling pathway. (A) mRNA changes in PIK3CA, PIK3CB, AKT and EGFR upon upregulation or downregulation of eIF6 in OSCC cells. (B) Western blot analysis of EGFR-AKT pathway-related markers after the increase of eIF6 in HN4 and HN6 cells. (C) Inhibition of eIF6 caused a visible reduction in p-EGFR, PI3K, and p-AKT protein expression. (D–F) LY294002 decreased the oncogenic phenotype of eIF6 by depressing proliferative (D), invasive (E) and migrative (F) ability. (G) Western blotting analysis in cells transfected with eIF6 or NC plasmids with or without the treatment of LY294002. All error bar values represent the SD. *P < 0.05, **P < 0.01, ***P < 0.001