Fig. 6

E2-induced ERα and TOP2β-dependent TFE3 breaks in translocation sites of ten Xp11.2 tRCC patients. A Statistics of TOP2β ChIP-qPCR results performed using primers designed for partner genes (NONO, PRCC, SFPQ, ASPSCR1 and MED15) in ten Xp11.2 tRCC patients in HK-2 cells under 0.1% DMSO (solvent-only control) or 100 μM etoposide treatment for 1 h. B Statistics of TOP2β ChIP-qPCR results performed using primers designed for TFE3 in ten Xp11.2 tRCC patients in HK-2 cells under 0.1% DMSO (solvent-only control) or 100 μM etoposide treatment for 1 h. C Statistics of ERα ChIP-qPCR results performed using the same primers as in panel A in HK-2 cells under 0.1% DMSO (solvent-only control) or 10 nM E2 treatment for 48 h. D Statistics of ERα ChIP-qPCR results performed using the same primers as in panel B in HK-2 cells under 0.1% DMSO (solvent-only control) or 10 nM E2 treatment for 48 h. ChIP-qPCR was normalized to Input DNA, experiments were repeated three times and data shown are means ± SD. Error bars indicate 95% confidence intervals (***p < 0.001)