Fig. 4
From: Identification of Desmoglein-2 as a novel target of Helicobacter pylori HtrA in epithelial cells
HpHtrA cleaves Desmoglein-2 on the cell surface of gastric epithelial cells independently of MMPs and ADAM10. NCI-N87 and MKN28 cells were infected with H. pylori P12 wt, N6 wt and N6 ΔhtrA for 24 h or left uninfected (mock). Where indicated, cells were treated with broad range MMP inhibitor Batimastat and ADAM10 inhibitor GI254023X (Bat/GI). A Whole cell lysates of infected NCI-N87 (left panel) and MKN28 cells (right panel) were analyzed by Western blot for full length hDsg2 (hDsg2FL). CagA, HpHtrA, and GAPDH were detected as loading controls. B Full length hDsg2 (hDsg2FL) of infected NCI-N87 cells (left panel) and infected MKN28 cells (right panel) was quantified and normalized to respective GAPDH loading controls. Data represent mean values ± S.D (n = 4). Asterisks indicate statistically significant differences (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant)