Fig. 3

Beclin1 overexpression regulates autophagic flux in CMECs. A–D Beclin-1 and LC3 expression in Len-GFP and Len-Becn1 CMECs treated with or without Bafilomycin A1 (Baf, 50 nM) was quantified by western blotting. Data are expressed as mean ± SD (n = 3). ^*P < 0.05 vs. Len-GFP group. E Endogenous LC3 expression in Len-GFP and Len-Becn1 CMECs treated with or without Bafilomycin A1 (50 nM). F Quantification of LC3-positive vesicles in CMECs. Data are expressed as mean ± SD (n = 3). ^*P < 0.05 vs. Len-GFP group, ^**P < 0.05 vs. Len-GFP + Baf group