Fig. 1

Beclin1 overexpression alleviates myocardial reperfusion injury in mice. A Survival analysis for mice subjected to 45 min ischemia and following 6 h perfusion (^*P < 0.05 vs. Sham group, ^** P < 0.05 vs. WT-I/R group, log-rank test for survival analysis). B Serum concentrations of LDH and CK in each group were measured using automatic biochemical analyzer. Data are expressed as mean ± SD (n = 5 for each). ^*P < 0.05 vs. WT-I/R group, ^**P < 0.05 vs. BECN1-Tg group, ^***P < 0.05 vs. WT group. C Representative TTC-Evan's Blue stained sections of heart from each group. Brick red-stained area represents the area at risk (AAR), whereas the white area indicates the infarcted size (IS). D Ratio of IS/LV, AAR/LV. Data are expressed as mean ± SD (n = 10 for each). ^*P < 0.05 vs. WT group, ^**P < 0.05 vs. WT-I/R group. E Effect of Becn1-Tg on no-reflow size and microvascular leakage. F Representative cross-sectional images of the left ventricular (LV) showing no-reflow regions indicated by the absence of thioflavin-S fluorescence within the ischemic area that consists of both no-reflow (white arrows) and reflow zones (yellow arrows) following IR. G Data are expressed as absorbent units per gram LV (absorbance/g) (n = 10 for each). ^*P < 0.05 vs. WT-I/R group. H Ratio of no reflow area/LV. Data are expressed as mean ± SD (n = 10 for each). ^*P < 0.05 vs. WT-I/R group