Fig. 6
From: Perturbation of PI3K/Akt signaling affected autophagy modulation in dystrophin-deficient myoblasts
Expression of autophagy-related proteins is highly increased in differentiating dystrophin-deficient myoblasts. Myoblasts were cultured in GM until 80–90% confluent and culturing in DM for 10 days prior to total protein extraction and immunoblot analysis. a Immunoblot analysis of Beclin1, Atg5, and Atg7 expression with α-tubulin expression as a loading control. Densitometry analysis representing b Beclin1, c Atg5, and d Atg7 expression. The graphs represent an average of four repeats from different samples. All data are presented as mean ± S.D