Fig. 5
From: Perturbation of PI3K/Akt signaling affected autophagy modulation in dystrophin-deficient myoblasts
FoxO3a is highly increased and predominantly localized in differentiating dystrophin-deficient myoblasts. Myoblasts were cultured in GM until 80–90% confluent and culturing in DM for 10 days before total protein extraction and immunoblot analysis with antibodies recognizing FoxO3a. a Immunoblot analysis of proteins during myoblast differentiation with α-tubulin expression as a loading control. Densitometry analysis representing b FoxO3a expression. Subcellular protein extraction was performed at the indicated time points using the REAP protocol before immunoblotting with an antibody which recognizes FoxO3a. c Immunoblot of the proteins during myoblast differentiation with α-tubulin and SSRP1 expression as loading controls. Densitometry analysis representing d FoxO3a expression in the cytoplasm and nucleus. The graph represents an average of three repeats from different samples. All data are presented as mean ± S.D. W: whole cell lysate; C: cytoplasm; N: nucleus