Fig. 4
From: Perturbation of PI3K/Akt signaling affected autophagy modulation in dystrophin-deficient myoblasts
Rictor-mTORC2 activation is lower in dystrophin-deficient myoblasts. Myoblasts were cultured in GM until 80–90% confluent and culturing in DM for 10 days prior to total protein extraction and immunoblot analysis with antibodies recognizing phospho-rictor (Thr1135), rictor, phospho-mTOR (Ser2448), and mTOR proteins. a Immunoblot analysis during myoblast differentiation with α-tubulin expression as a loading control. Densitometry analyses of b total rictor expression c rictor activity d total mTOR expression, and e mTOR activity. The graphs represent an average of three repeats from different samples. All data are presented as mean ± S.D