Fig. 2
From: Perturbation of PI3K/Akt signaling affected autophagy modulation in dystrophin-deficient myoblasts
PTEN expression is higher while PI3K activation is decreased in dystrophin-deficient myoblasts. Myoblasts were cultured in GM until 80 to 90% confluent and culturing in DM for 10 days before total protein extraction and immunoblot analysis. a Immunoblot analysis of PTEN, phospho-PI3K (p85), and total PI3K during myoblasts differentiation with the α-tubulin expression as a loading control. Densitometry analysis of b PTEN expression, c total PI3K expression, and d PI3K activity. The graphs represent an average of three repeats from different samples. All data are presented as mean ± S.D